Immunohistochemistry
Read the topic background here, then explore the labeled visual and structured learning explanations on this page.
Read explanation on this page ↓ See diagram ↓Immunohistochemistry — on-site reading
An introductory overview for this topic. The article introduction is reproduced here, so you do not need to leave MedAtlas to read it. It may not match the latest official medical guidance.
Immunohistochemistry is a form of immunostaining. It involves the process of selectively identifying antigens in cells and tissue, by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Albert Hewett Coons, Ernest Berliner, Norman Jones and Hugh J Creech was the first to develop immunofluorescence in 1941. This led to the later development of immunohistochemistry.
Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. In some cancer cells certain tumor antigens are expressed which make it possible to detect. Immunohistochemistry is also widely used in basic research, to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.
How this connects to MD Pathology
To study this topic responsibly, identify its normal structure or function, distinguish the main mechanism from its observable consequences, and ask which data can test a competing explanation. The subject foundations below outline these connections without pretending to be a specialist textbook chapter.
Text credit: Wikipedia contributors, “Immunohistochemistry”, original article · authors & revision history · CC BY-SA 4.0. Unmodified opening extract, accessed 24 September 2026. This Wikipedia-derived section is provided under CC BY-SA 4.0; the independent MedAtlas notes and design are separate works.
Immunohistochemistry · visual study map
Scalable vector illustration. Labeled conceptual map, not a precise anatomical, histological or diagnostic image.The wording in this learning map is adapted from the attributed Wikipedia background section below (CC BY-SA 4.0).
What the underlying subject studies
At postgraduate and higher-specialty level, begin with normal anatomy and physiology of the relevant organ system, then compare distinct disease mechanisms, their evidence base and the limitations of available investigations.
How mechanisms and evidence connect
Advanced study requires evidence appraisal, multidisciplinary interpretation and a clear distinction between established facts, hypotheses and research findings. Procedural, diagnostic and prescribing skills must be learned under an accredited program.
How to develop a sound explanation
Identify the scope of this topic within the named specialty, connect it to the applicable patient population and formulate a structured question that can be answered using current specialty literature and supervised teaching.
References and verification (optional)
All reading material on this page appears above. The links below are for checking the primary syllabus, research or source attribution, not requirements for opening this lesson.